Giorgo,
We have done that routinely for quite some time now. We had problems
when using a normal CCD detector, where we had to collect two or three
sweeps to avoid overloads (see below). Since we have the PILATUS this is
not necessary anymore and the data behaves fine. "Problems" still
persisting are: we have only a single axis goniometer, which can lead to
low completeness in P1 and P-1. Highest energy (17keV) and closest
distance (188mm) at our beamline have many SM crystals (even the ones
that "don't diffract" in house -- that is a 300 or 500 u sealed tube)
with an I/sig of 5-10 at the edge of the detector. Crunch, Acorn,
ShelxCDE and ShelxS don't have any problem with any of the data we
collected to <0.9A resolution. The multipass caused some inexplicable
non definite positives during refinement. We haven't tracked that down
systematically, so it might just have happened haphazardly.
HTH,
Jens
On Wed, 2012-02-08 at 11:41 +0000, Giorgio Giardina wrote:
> Hello,
> I have some interesting small molecule xtals.
> I was wondering if it is possible to collect a small molecule data-set using a sincrotron macromolecular xtallography beam line, maybe with a very low beam intensity and moving the detector as close as possible?
> Has anybody experienced that?
> And if I get the images back home, can I process them using standard macromolecular software or do I need ab-initio special programs?
> Will MR work for phasing?
>
> Thanks in advance,
> Giorgio
|