This paper is a favorite: http://www.ncbi.nlm.nih.gov/pubmed/2160019
J Mol Biol. 1990 May 5;213(1):159-66.
Crystallization of Escherichia coli catabolite gene activator protein with its DNA binding site. The use of modular DNA.
On Feb 15, 2012, at 12:06 AM, LISA wrote:
> Hi all,
>
> I have a DNA binding protein. I can get crystals when I mix 8-28 nt dsDNA
> with my protein. But neither of them has good diffraction. Some biochemical
> data said the longer of DNA, the tigher of the binding betwwen DNA and my
> protein. The binding is not sequence-specfic. Does anyone have suggestion
> of the optimization? What is the good length of DNA for crystallization?
> Thank you.
>
> Lisa
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