When you crystallized your lysozyme, did you have any other anomalous scattering atoms in the conditions? I am thinking of chloride ions in particular, but there could be many others. How did you distinguish between sites of xenon and sites of other kinds of atoms in your analysis?
Did you do a control experiment without xenon? Maybe with air? Maybe with and without pressurization?
Jim
________________________________________
From: CCP4 bulletin board [[log in to unmask]] on behalf of Brennan Bonnet [[log in to unmask]]
Sent: Thursday, February 02, 2012 10:43 AM
To: [log in to unmask]
Subject: [ccp4bb] Lysozyme/Xenon derivatives & Phenix.
Hi all,
I have a strange result using Phenix's AutoSol to look for xenon sites in lysozyme.
....
|