One last thing--sometimes crystals can be frozen as is, particularly
if you use mitegen mounts and get nearly all of the mother liquor off
the crystals by dabbing the loop on the dry surface next to the drop
several times. So simple it is always worth a try....
JPK
On Tue, Feb 7, 2012 at 2:37 AM, Mark J van Raaij <[log in to unmask]> wrote:
> Rationalising it completely may only be possible once you know the nature of the crystal contacts, i.e. when you have solved the structure. Until then it is mainly a matter of experimenting.
>>
>> -----Original Message-----
>> From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of
>> Theresa H. Hsu
>> Sent: Monday, February 06, 2012 11:00 PM
>> To: [log in to unmask]
>> Subject: Re: [ccp4bb] Freezing crystal
>>
>> Hi all
>>
>> Thanks for all the suggestions which I will try soon.
>>
>> How do the crystallization condition (PEG vs. salts like ammonium
>> sulfate) affect the croyprotectant condition? Do factors like presence
>> of low concentration of high molecular weight PEG (> 2000) mean PEG is
>> better? Do buffers and salts in protein also important?
>>
>> Trying to rationalize it :)
>>
>> Theresa
--
*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
email: [log in to unmask]
*******************************************
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