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Subject:
Re: Off topic: His-tag purification
From:
Luca Jovine <[log in to unmask]>
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Date:
Mon, 16 Jan 2012 18:36:31 +0000
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…alternatively, depending on what is the source of your protein, you might have to dialyze the (concentrated) sample before applying it to IMAC. Insect cell media, in particular, can be pretty good at stripping off Ni2+ from IMAC supports. HTH, Luca
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