Hi,
I'm sure there are proteins that were crystallized at low pH but I can't remember which. The best thing is to go to the BMCD database: http://xpdb.nist.gov:8060/BMCD4/index.faces
and query it with the key pH (look into advanced search).
Cheers,
Boaz
Boaz Shaanan, Ph.D.
Dept. of Life Sciences
Ben-Gurion University of the Negev
Beer-Sheva 84105
Israel
E-mail: [log in to unmask]
Phone: 972-8-647-2220 Skype: boaz.shaanan
Fax: 972-8-647-2992 or 972-8-646-1710
________________________________________
From: CCP4 bulletin board [[log in to unmask]] on behalf of Sam Arnosti [[log in to unmask]]
Sent: Monday, November 07, 2011 7:19 AM
To: [log in to unmask]
Subject: [ccp4bb] Crystalization in low PH
Hi everyone
I have a protein that is extraordinarily stable at PH=3.0 or even 2.0.
I want to crystallize it in the low PH and compare the differences between the crystals in regular PH and low PH.
I was wondering how people set up the boxes in low PH, as usual buffers are mostly less acidic.
Regards
Sam
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