Which RNA polymerase are you working?
xiaopeng
----- 原始邮件 -----
发件人: "Appu kumar" <[log in to unmask]>
收件人: [log in to unmask]
发送时间: 星期五, 2011年 11 月 25日 上午 2:04:39
主题: [ccp4bb] RNA polymerase purification
Hello every one,
Sorry for asking off topic question, I have purified different subunit of RNA polymerase, now i want to assemble it. Can you give me valuable suggestion regarding the gel filtration column and ion exchange column which will facilitate the purification of assembled RNA pol. I want to know name of gelfilteration column and other column which would be used to purify. I do not hav much knowledge regarding column so would you please help me by giving me your valuable suggestion.
Your suggestion would be highly appreciated.
Thank you.
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