The is probably the cleanest and fastest way of greasing plates.
Take a 15ml Falcon tube and unscrew the cap. You will notice that the
cap is exactly the width as the well of the VDX plate. Using a fairly
wide bored metal syringe needle, pierce 8 holes into the cap from the
outside, along the top edge of the cap. Fill the Falcon tube with
Vaseline and screw the cap back onto the tube. Loosen the cap about 2 to
2.5 turns, just before it comes of the tube. Stick the tube in a heating
block, wait for the Vaseline to melt. When you now invert the tube with
the liquid Vaseline, it should not come running out, but if you press
the top of the cap against the well of the VDX plate, you will see that
a small amount of Vaseline is let out through the small holes. If you
lift the tube off the well, air will then go into the tube and you can
press the cap against the next well. Continue until you're. Should take
no more than a few seconds to do the whole plate.
Cheers,
Chris Browning
--
Dr. Christopher Browning
Post-Doctor to Prof. Petr Leiman
EPFL
BSP-416
1015 Lausanne
Tel: 0041 (0) 02 16 93 04 40
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