Hi,
With the electronic Biohit 8 channel pipette you can set up 3x96 drops in 5 minutes. Lowest volume is 0.2 ul. We have used this system for a couple of years with RNase free filter tips for RNA-protein complexes. With the recent arrival of a shared Mosquito we have used the Biohit less but it is still convenient to have the setup. You need good eyesight and a steady hand, though.
Cheers,
Martin
On Nov 18, 2011, at 3:35 PM, "xaravich ivan" <[log in to unmask]> wrote:
> Hi,
> I apologize in advance as it is not a ccp4 related question, but over the years, CCP4bb is synonymous with protein crystallographers virtual university, at least for me.
>
> Ok, now I do not have an easy access to crystallization robot, so I was hoping if someone here have ever used the 96 well plates for manually setting drops with much lower solution/sample volumes (0.1-0.2micro litres).
>
> I heard about a clicker syringe that can be used to manually add lesser volumes but I am not sure how to go about it.
> Please let me know if you are aware of or are routinely setting 96 well trays with much lower volumes of crystallization solutions as well as samples, manually.
>
> thanks in advance,
> ivan
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