Vaseline (pure, with no additives) from CVS/Walgreens in a 10 ml syringe
and a pipette tip (wide bore) also works very well.
On 11/18/11 12:58 PM, Bosch, Juergen wrote:
> Dow Corning Heavy Vacuum Grease, a 10 ml syringe and a shorter cut 200
> µl tip attached to the syringe.
>
> Jürgen
>
> On Nov 18, 2011, at 12:55 PM, Dima Klenchin wrote:
>
>> Hello,
>>
>> I wonder what everyone is using for sealing hanging drop slides on VDX
>> plates? For the most part, we paraffin oil but I am unhappy with it
>> because
>> it is too think and too frequently there is break in the oil and the
>> drop
>> dries too much. I find vacuum grease to be not terribly practical
>> because
>> it takes too much time - particularly when the well needs to be opened
>> (seeding, modifying well content, etc).
>>
>> In ideal world I would like to find a much thicker oil that 1)
>> contains as
>> little volatiles as paraffin oil, and 2) allows no more water vapor
>> diffusion through it than paraffin oil. Hampton suggests Cargille
>> immersion
>> oils for this purpose but the MSDS for these oils states that they have
>> slight odor, so I am a bit concerned with unknown volatiles getting into
>> crystallization drop.
>>
>> So, what do you like to use? Thanks much,
>>
>> - Dima
>
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