Hello,
i am a master student, and i am doing my thesis in protein folding/unfolding. This is the first time i used ccpnmr and i am trying to make my backbone assignment. I introduce my sequence, already assigned in HSQC and 3D spectra all H and N shifts and when trying to do protein sequence assignment, i have chose the spin system that already know that is my final residue (94 Gly), the software in the sequence location does not correlate with it. I don know if i am doing something wrong and i was hoping some advice or hint about my problem.
Thanks everyone
Daniel
|