Hello,
Tim says:
Menu:Assignment:Automated Seq. Assignment
- Set "Use?" for spectra in Settings tab as required
- Press [Find Resonances From Peaks] in Spin Systems tab
Otherwise the semi-automatic (Assignment:Protein Sequence Assignment)
system will add non-amide resonances as it goes. These can be typed with
[Add HA/CO/CA/CB].
Wayne
On Thu, 6 Oct 2011, Kovilen Sawmynaden wrote:
> Dear all,
>
> I have recordered 4 standard backbone (triple resonance) experiments: HNCACB, CBCA(CO)NH, HNCO and HN(CA)CO.
>
> I have linked all these (i.e. carbon resonaces) to my 15N HSQC/anonymous NH spin systems.
>
> In order to run the automated sequence tool (or even use the Format Convertor to output to MARS) should I explicitly tell Analysis "this is a CB" or "CA" i.e. also manually set amino acid TYPES.
>
> Conceptually, I can see I would need to. But, if I am essentially duplicating peaks (i.e. by picking a NEW carbon resonance in each spectra) would I be making it worse by feeding too many identiacl resonaces into the algorithm?
>
> Any advice would be really helpful,
>
>
> Thanks.
>
>
> Kovilen (PhD protein NMR).
>
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