Another possibility (other than those already mentioned) is to try
freezing without a cryoprotectant, by fishing the crystals out onto a
mesh and removing all the mother liquor.
The following paper has some details:
"Direct cryocooling of naked crystals: are cryoprotection agents
always necessary?"
Erika Pellegrini, Dario Pianoa, and Matthew W. Bowlera
Acta Cryst. (2011). D67, 902–906
--
Andrew Purkiss
X-ray Laboratory Manager
Cancer Research UK
London Research Institute.
Quoting Leonard Thomas <[log in to unmask]>:
> Hi All,
>
> I have run into a very sensitive crystals system when it comes to
> cryo protecting them. I have run through the usual suspects and
> trays are going to be setup with a cryo protectant as part of
> crystallization cocktail. The one problem that seems to be
> occurring is that the crystals crack as soon as they are transfered
> out of the original drop. I am running out of ideas and really
> would love some new ones.
>
> Thanks in advance.
>
> Len
>
> Leonard Thomas Ph.D.
> Macromolecular Crystallography Laboratory Manager
> University of Oklahoma
> Department of Chemistry and Biochemistry
> Stephenson Life Sciences Research Center
> 101 Stephenson Parkway
> Norman, OK 73019-5251
>
> [log in to unmask]
> http://barlywine.chem.ou.edu
> Office: (405)325-1126
> Lab: (405)325-7571
>
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