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Dear Len,
just to be on the safe side, my list of 'usual suspects' includes
- - glycerol/PEG400
- - LiCl et al at high concentration
- - Butanediol
- - sugars (glucose/ fructose)
- - oil
- - NaMalonate
- - MPD
...
you mention cracking upon transferring the crystal.
- - do you use a pipet for transfer?
- - addition of cryo TO the drop?
- - did you try slow (several minutes - 1hr) / quick addition of cryo
protectant
- - seeding into slightly different conditions/additive screens
- - seeding into cryo conditions
...
How about collecting data at room temperature?
Hope this list contains some new ideas.
Best wishes,
Tim
On 10/26/2011 06:46 PM, Leonard Thomas wrote:
> Hi All,
>
> I have run into a very sensitive crystals system when it comes to cryo
> protecting them. I have run through the usual suspects and trays are
> going to be setup with a cryo protectant as part of crystallization
> cocktail. The one problem that seems to be occurring is that the
> crystals crack as soon as they are transfered out of the original drop.
> I am running out of ideas and really would love some new ones.
>
> Thanks in advance.
>
> Len
>
> Leonard Thomas Ph.D.
> Macromolecular Crystallography Laboratory Manager
> University of Oklahoma
> Department of Chemistry and Biochemistry
> Stephenson Life Sciences Research Center
> 101 Stephenson Parkway
> Norman, OK 73019-5251
>
> [log in to unmask]
> http://barlywine.chem.ou.edu
> Office: (405)325-1126
> Lab: (405)325-7571
>
- --
- --
Dr Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
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