Are you absolutely sure of the spacegroup?
Eleanor
On 09/13/2011 09:55 AM, #HEW KAI LI KELLY# wrote:
> Hi,
>
> I am facing some problems in solving my structure now, so I am wondering if anyone is able to give me any tips and tricks on this matter.
>
> My protein-DNA complex structure diffracted to 1.5A. There are 4 missing residues, 2 on each terminal. There is no twinning in the data. The angles, the bonds, the rotamers and the Ramachandran plot are okay too. I am using molecular replacement for the phasing and the sequence homology between my protein and my homologous model is 33%. The electron density map for the protein looks very nice and there is also nice density for the DNA. Rfree converged from the initial 39%. However, Rfree refused to go down any further and it's still around 30-31%. Does anyone have any suggestions for me? Thank you in advance!
>
> Warmest Regards,
> Kelly Hew
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