Hello,
Tim says;
Make whichever feels most useful and use it in the normal way. I would try
HCC-TOCSY-NH for starters. To get it to work you should select it like any
other match/query peak list.
Wayne
On Thu, 11 Aug 2011, S.P. Skinner wrote:
> Hi Wayne
>
> Which sort of synthetic list do I need to create and how can I then get such
> a list to work with semi-automated assignment?
>
> On 08/11/2011 12:16 PM, Wayne Boucher wrote:
>> Hello,
>>
>> Tim says:
>>
>> Already there. From the right mouse menu use "Assign:Unite resonance
>> positions" on the selected peak. This will line up all the peaks with the
>> same resonance assignment (on each dim separately) in that spectrum.
>>
>> Re the second point, only amide based spectra are used in the automated
>> (and semi-automated) assignment routines. To get round this a synthetic
>> HNCACB peak list could be made, although at present this requires that
>> there is an experiment of HNCACB type present (this could actually be a
>> duplicate of some other experiment, only the type is important). At some
>> stage we will improve things by adding the ability of making synthetic peak
>> lists in new, blank spectra.
>>
>> Wayne
>>
>> On Thu, 11 Aug 2011, S.P. Skinner wrote:
>>
>>> Hi All,
>>>
>>> Is is possible to have a feature wherein duplicate peaks in difference
>>> planes of a TOCSY are lined up, the same was as for side chain amides in
>>> an HSQC. I have a CHD2 detected CC TOCSY, such that for I, L and V there
>>> are two sets of peaks in different planes and I would like to line them up
>>> automatically as opposed to manually going through the strips.
>>>
>>> On a related note, can such a TOCSY be used as a query spectrum in the
>>> automated assignment window, when matched to a CCCcoNH or an HNcoCACB?
>>>
>>> Kind Regards
>>>
>>> Simon Skinner
>>>
>>> --
>>> Simon P Skinner
>>> Protein Chemistry Group
>>> Leiden Institute of Chemistry, Universiteit Leiden
>>> Phone: +31 71 527 6089 / Fax: +31 71 527 4349
>>> E-mail : [log in to unmask]
>>>
>
> --
> Simon P Skinner
> Protein Chemistry Group
> Leiden Institute of Chemistry, Universiteit Leiden
> Phone: +31 71 527 6089 / Fax: +31 71 527 4349
> E-mail : [log in to unmask]
>
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