Hi Anita,
Won't harm if you put it on crystallization tray. You never know what
these proteins might do.
Allan
Quoting Zheng Zhou <[log in to unmask]>:
> Hi, Anita
>
> If you could find a way to test the elute's activity/binding to its'
> substrat/cofactor, then you will learn much more about your target. If the
> function assay is elusive, you could try superose column (5KDa-5MKDa). Does
> your light scattering tell you about the estimated size and MW?
>
> Best,
>
> Joe
>
> On Sat, Aug 27, 2011 at 1:29 AM, anita p <[log in to unmask]> wrote:
>
>> Hi Yury,
>> I have done dynamic light scattering and it shows its polydispersed.
>> Please let me know if it is still ok for setting trays.
>> reg.
>> anita
>>
>> On Fri, Aug 26, 2011 at 10:21 PM, Yuriy Patskovsky <
>> [log in to unmask]> wrote:
>>
>>> Anita,
>>> an assembly may be quite large - I would check it somehow, maybe by light
>>> scattering or centrifugation
>>>
>>> Good luck
>>>
>>> Yury
>>> ------------------------------
>>> *From:* CCP4 bulletin board [[log in to unmask]] on behalf of anita p
>>> [[log in to unmask]]
>>> *Sent:* Friday, August 26, 2011 3:03 AM
>>> *To:* [log in to unmask]
>>> *Subject:* [ccp4bb] Protein aggregation and crystallization
>>>
>>> Hi All,
>>> I am working on a protein which has a membrane spanning region and as
>>> cytosolic domain.I have made various deletion constructs of the
>>> protein, so
>>> that I can have a crystallizable fragment. There is no homologues
>>> mentioned
>>> in the pdb for this protein.
>>> All of these constructs are purified successfully but when concentrated
>>> and loaded on a gel filtration column Superdex-200, they elute in the void
>>> volume. But the proteins donot precipitate out.... !!
>>> Is it worth while to go ahead for crystallization trials??
>>> Any other suggestion is most welcome.
>>> Thanks
>>> Anita
>>>
>>>
>>
>
--
Allan Pang
PhD Student
G35 Joseph Priestley Building
Queen Mary University of London
London
E1 4NS
Phone number: 02078828480
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