Hi Opher,
I have a project where a protein was made 'cysless' and selective cysteines were introduced to allow for directed crosslinking using MTS and aldrithiol activated reagents (see Toronto Research Company and Pierce for a range of reagents of different lengths). A side product of these crosslinks was a dramatic improvement in crystal diffraction.
Katherine Rank
Rayment Lab
On 08/15/11, Opher Gileadi wrote:
> Hi all
>
> Does anyone have experience or insights on intramolecular crosslinking of proteins before crystallization? The idea is to lock a felxible (multidomain) protein into a restricted conformation.
>
> Thanks,
> Opher
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