Dear David,
Thanks for the extra data. The crucial bit was:
> My data is 3 dimensional with a C H and N axes. The N and H are directly
> bonded. I have set the full type experiment type to h[C]_H[N].NOESY.
Analysis 2.1.5 does not support this kind of indirect NOE transfer.
We recommend that you change over to Analysis 2.2, that does support
indirect NOE transfer. Some time this summer we shall set Analysis 2.1.5
to deprecated, and upgrade Analysis 2.2 to stable, so you should be doing
it soon anyway.
YOurs,
Rasmus
---------------------------------------------------------------------------
Dr. Rasmus H. Fogh Email: [log in to unmask]
Dept. of Biochemistry, University of Cambridge,
80 Tennis Court Road, Cambridge CB2 1GA, UK. FAX (01223)766002
On Mon, 11 Jul 2011, Rasmus Fogh wrote:
> Dear David,
>
> It is hard to answer without more information.
>
> What are the Full Type of the experiments (Look in M:Experiments:Experiment
> Types)? Do they look correct, and do they mention through-space (or NOESY)
> transfer?
>
> What version of Analysis are you using?
>
> Have you done an update, so you have the latest patches?
>
> What happens when you try to make restraints? An Error, the Experiment does
> not apear as an option, nothing happens, ...?
>
> If these questions do not by themselves suggest an answer, you might hve to
> send us the Analysis project to work on (off list, please, you can send to me
> directly).
>
> We may be a bit slow this week as Wayne is going on holiday and I shall be
> way till Thursday.
>
> Yours,
>
> Rasmus
>
> ---------------------------------------------------------------------------
> Dr. Rasmus H. Fogh Email: [log in to unmask]
> Dept. of Biochemistry, University of Cambridge,
> 80 Tennis Court Road, Cambridge CB2 1GA, UK. FAX (01223)766002
>
> On Fri, 8 Jul 2011, David Langelaan wrote:
>
>> Hello,
>>
>> I am trying to make distance restraints from several of my spectra through
>> the Structure-Make Distance Restraints menu.
>>
>> I have 3 experiment types which I have assigned peaks for, a 15N
>> NOESY-HSQC, a 13C NOESY-HSQC and a 15N,13C HSQC-NOESY-HSQC.
>>
>> I am able to make distance restraints without trouble for the first 2
>> experiments by clicking the Make assigned restraints button, but this does
>> not work for the 15N,13C HSQC-NOESY-HSQC experiment even though I have
>> peaks assigned.
>>
>> Is this a bug of some sort, or am I doing something wrong?
>>
>> Thanks,
>>
>> David Langelaan
>>
>
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