Please clarify:
Did you process the sets separately from the images?
Or did you process 360 degrees of images all the way to scaled, but
unaveraged results, then average reflections from the 5 different rotation
ranges?
Or something else?
With most processing programs that I am aware of, one can adjust their
sigmas to anything they want to. What happens when you use the same
adjustments for the 180 degrees that you did for the others?
-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of
Vennila Natesan
Sent: Sunday, July 10, 2011 7:54 AM
To: [log in to unmask]
Subject: [ccp4bb] abnormal I/Sigma over phi rotation range
Dear CCP4BB users,
In order to determine the redundancy at which the structure can be solved, I
divided the master data set of my protein into five sets with phi rotations
of 45,65,90, 180 and 360 degrees. I got the mean I/Sigma value as
22.4(11.4), 21.7(11.0), 22.7(11.2), 60.9(45.2) and 15.6(8.1) respectively.
I will be very helpful if i get any idea/possible reasons for the abnormal
value for 180 degree dataset.
For the information, the completeness values are
75.7(78.5),92.6(92.7),99.5(97.7),99.6(97.7)and 99.5(96.4) respectively and
values inside brackets are for highest resolution shell. There was no
noticable change in mosaicity
value. The Rmerge values are 2.2, 2.4, 2.7, 3.2, and 5.7 (8.7)
respectively.
Thanks in advance
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