hey Mischa
I would guess that is a split cobalt/metal site occupancy 0.1 and 0.9 or something like that.
If you calculate an anomalous difference map you may be able to confirm/reject that suggestion, depending on the strength of the anomalous signal.
cheers
Preben
On 07.07.2011, at 17:07, Machius, Mischa Christian wrote:
> Y'all,
>
> I was wondering if anyone had any thoughts about a feature we observe with a metal-binding site: we have a cobalt that is bound by four histidines and one carboxyl group. There is extra density near the cobalt. See pictures below. The extra density spans the NE2 atoms from two histidines. The Fo-Fc peak (green) has a height of up to 10 sigma (eight molecules in the asymmetric unit, all showing the same feature).
>
> I placed a water molecule into the density to get some distances: the distances between the peak and the neighboring histidine NE2 atoms is ~1.8Å and ~2.0Å, resp. The distance between the peak and the cobalt is ~1.7Å. The resolution is 1.24Å.
>
> Any input would be greatly appreciated.
>
> Many thanks in advance!
>
> Cheers!
> MM
>
>
> <Screen shot 2011-07-07 at 9.44.43 AM.png><ATT00001.c><Screen shot 2011-07-07 at 9.44.55 AM.png>
J. Preben Morth, Ph.D
Group Leader
Membrane Transport Group
Nordic EMBL Partnership
Centre for Molecular Medicine Norway (NCMM)
University of Oslo
P.O.Box 1137 Blindern
0318 Oslo, Norway
Email: [log in to unmask]
Tel: +47 2284 0794
http://www.jpmorth.dk
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