Does anyone out there have a protocol of growing HEWL crystals that are
all 50-100 microns wide? I gave this project to a summer student
recently, thinking it would be easy, but it is turning out to be more
difficult than I thought. Keep getting sphereulites instead of small
crystals. Yes, I know you can smash a large lysozyme crystal with a
hammer, but that is not exactly what I was going for. What I was hoping
for was a well-defined protocol for growing "reference" crystals that
stay evenly illuminated in our x-ray beams as they rotate. The beam is
100 um wide.
I'm sure someone has done this before?
-James Holton
MAD Scientist
|