You could try loading a small 1ml HiTrap (or similar) Q or S column with your protein - and knocking it off it in one go with high salt, alternatively micro-dialysis against a solution containing PEGs can also work well.
Tony
Sent from my iPhone
On 21 Jul 2011, at 17:54, "Narayanan Ramasubbu" <[log in to unmask]> wrote:
> Dear All:
> We have been trying to crystallize a protein which is large - > 100 kDa. This is soluble but the best we can get is about 1 mg/mL.
> It did crystallize but did not diffract well. Efforts to increase the concentration has been unsuccessful. I am wondering whether there are methods that others use to increase the concentration other that using amicon columns.
> Any help will be appreciated.
> Thanks
> Subbu
|