Hi Brittney,
DNA is pretty standard so the restraints should be in the dictionary. Perhaps the DNA in your model has non-standard residue names (PDBv2). Are your bases called DT, DA, etc? Do your atom names have "*" or "'"?
Cheers,
Robbie
________________________________
> Date: Tue, 26 Jul 2011 12:45:08 -0400
> From: [log in to unmask]
> Subject: [ccp4bb] Creating a non-minimal mmCIF dictionary for DNA?
> To: [log in to unmask]
>
> I am trying to refine a protein-DNA structure, and I am having
> difficulties refining the DNA using COOT. I used the ccp4i Phaser
> program to molecular replace the protein and DNA simultaneously (there
> are solved crystal structures of the free protein and a similar DNA
> bound to another protein), but I am only able to manually refine the
> protein in COOT. If I try to refine the DNA, a window pops up with the
> following statement: No Restraints Found! Non-existent or minimal
> description of restrained residues. How do I create a non-minimal mmCIF
> dictionary for just the DNA? I am relatively new to crystallography
> and would appreciate any guidance.
>
> Brittney
>
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