Dear All:
We have been trying to crystallize a protein which is large - > 100 kDa.
This is soluble but the best we can get is about 1 mg/mL.
It did crystallize but did not diffract well. Efforts to increase the
concentration has been unsuccessful. I am wondering whether there are
methods that others use to increase the concentration other that using
amicon columns.
Any help will be appreciated.
Thanks
Subbu
|