On 06/24/2011 08:50 AM, mullapudi edukondalu wrote:
> Dear Members,
>
> I have my first data set on one of my protein crystals, that diffract to
> 2.7 A, and the space group is I222. According to Mathews coefficient, there
> should be 4 molecules in the asymmetric unit. But, when I run molecular
> replacement programme it found only 3, and the crystal packing is not that
> good. I have 100% similar model. And Ref mac gave me an Rfree of 46%. Could
> any one of you please suggest me how to go further with this problem?
> Looking forward to hear from you.
>
> Best Regards
>
> Edukondalu Mullapudi
>
I guess you tried I 212121 to as a possible spacegroup?
Eleanor
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