You do need to try space group I21 21 21 as well as these two space groups are indistinguishable from systematic absences
Phil
On 24 Jun 2011, at 08:50, mullapudi edukondalu wrote:
> Dear Members,
>
> I have my first data set on one of my protein crystals, that diffract to 2.7 A, and the space group is I222. According to Mathews coefficient, there should be 4 molecules in the asymmetric unit. But, when I run molecular replacement programme it found only 3, and the crystal packing is not that good. I have 100% similar model. And Ref mac gave me an Rfree of 46%. Could any one of you please suggest me how to go further with this problem? Looking forward to hear from you.
>
> Best Regards
>
> Edukondalu Mullapudi
>
> --
> Edukondalu Mullapudi
> PhD Student
> Molecular Host-Pathogen Interactions (MHPI)
> Division of Structural Biology (SB)
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