Dear Crystallographers,
what is the dogma with regard to affinities in crystals? For example,
if I soak three crystals in 1pM, 1nM, and 1uM compound X, and they all
show equivalent density, does that mean that the affinity is really
better than 1pM, or is the crystal of such a high local concentration
(~600mg/mL) that it will be fully occupied at nearly any
concentration, provided external ligand concentration does not change
due to binding in the crystal? I guess there is also the problem that
the crystallization solutions are very non-physiological, but
neglecting that, is there any straightforward way to think of this, or
is there a good reference?
Jacob Keller
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Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
cel: 773.608.9185
email: [log in to unmask]
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