Hi,
the best way which worked for me:
Open all the relaxation series (T1/T2) experiment in the ccpnmr project which already has your assigned HSQC (If you save it as a new project it is the best).
Reference the assigned HSQC in line with the relaxation HSQC's as best you can by selecting Experiment-->Spectra---> Referencing--->change the referencing numbers if say your sweep widths for the 3D assigned HSQC is different from your relaxation HSQC's.
Now picking and coping of the assignments.
Go to Peak menue
Click on the peak lists
select the assigned 3D HSQC and then click on copy peaks
a pop will come saying select the destination list and you select the first HSQC from your T1/T2 series (I will name it X).
All the assignments will be copied to experiment X.
Now select the assigned experiment X by using mouse and then press Alt+Ctrl+P.
This will rearrange all the peaks to the center.
Go back to Peak menu
Click on peak lists
Select experiment X and then select copy peaks
Select destination spectrum and this will copy the assignments to the second spectrum.
Its better to select every respective spectrum after copying of the peaks and then applying Alt+Ctrl+P to make sure that every cross peak is centered.
You can copy peak list to every HSQC in the relaxation series by using this.
After making sure that every peak is in the right place.
go to the Data analysis menu
select your first T1/T2 (shorted delay) in the reference peak list menu
select experiment series which you want to work on.
Try fitting function A exp (-Bx) somehow it gives you better fit then A exp(-Bx)+C
Uncheck pick new peaks, assign groups, skip zero merit peaks. By this you will not allow the program to work out the new peak positions or new intensities and will stick to your provided picked peaks (which made life easier for me).
Hope that helps!
Regards,
Masooma
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