Hi Wayne,
I set tolerances to zero for both, is that going to cause problems with
the algorithm? Should I just set them to something tiny?
On 05/27/2011 03:38 AM, Wayne Boucher wrote:
> Hello,
>
> So you mean that even with small tolerances it was still picking peaks
> away from where the reference peak position was? I just tried an
> example and it picked them on top of the reference peak (well, it
> looked like it was off 0.001 ppm in some cases) and it did not do this
> with normal tolerances (so in that case it picked at the extrema which
> were within the tolerance in my example).
>
> Wayne
>
> On Fri, 27 May 2011, Simon Skinner wrote:
>
>> Hi Wayne
>>
>> I have tried it with and without those options selected but i still
>> get the same result, is there a reason for this?
>> On May 26, 2011, at 6:12 PM, Wayne Boucher wrote:
>>
>>> Hello,
>>>
>>> Tim suggests that you should click the "Pick new peaks" and "Pick
>>> non-maxima peaks" check boxes. Now what that does is that it will
>>> try and find a local extremum within the specified tolerances and if
>>> it doesn't find one it will put down a peak at the reference
>>> position. So if you want all your peaks to be at the reference
>>> position then you should make the tolerances small enough that it
>>> will then not find any extrema. The tolerances are set in the table
>>> below those two check boxes, you might need to resize the dialog to
>>> see it.
>>>
>>> Wayne
>>>
>>> On Thu, 26 May 2011, S.P. Skinner wrote:
>>>
>>>> Hi All
>>>>
>>>> I am using the follow intensity changes function to calculate R1
>>>> and R2 rates for my protein, but there appears to be an issue
>>>> wherein the peak position changes from point to point. I have
>>>> picked and assigned the first plane, but not the others. Is there
>>>> a way to force the peak picking to stay in exactly the same place
>>>> or would I have to manually assign all planes of the pseudo 3D
>>>> spectra? If the latter is true, is there a quick way to do this?
>>>>
>>>> Regards
>>>>
>>>> Simon
>>>>
>>>> --
>>>> Simon P Skinner
>>>> Protein Chemistry Group
>>>> Leiden Institute of Chemistry, Universiteit Leiden
>>>> Phone: +31 71 527 6089 / Fax: +31 71 527 4349
>>>> E-mail : [log in to unmask]
>>
--
Simon P Skinner
Protein Chemistry Group
Leiden Institute of Chemistry, Universiteit Leiden
Phone: +31 71 527 6089 / Fax: +31 71 527 4349
E-mail : [log in to unmask]
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