Dear Chris,
I have not used this particular condition, but as a rule of thumb I use
"like with like", e.g. glycerol, ethylene glycol, PEG400 etc. with PEG
conditions, and saltlike compounds (sucrose, xylitol, salts) for salt
conditions. In your case I would try glucose or xylitol or just look
what happens if you increase the ammonium phosphate concentration to say
2M without adding glycerol.
Good luck!
Herman
-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of
Chris Ulens
Sent: Thursday, May 26, 2011 12:27 PM
To: [log in to unmask]
Subject: Re: [ccp4bb] recommendation for ammonium dihydrogen phosphate
cryo
Hi,
I would like to get recommendations for a proper cryo solution for a
crystallization hit from the Hampton crystal screen Ammonium di-
hydrogen phosphate 0.4M. I tried increasing glycerol up to 30% with the
same ammonium phosphate concentration or increasing glycerol up to 30%
in the presence of 1.3M ammonium phosphate. Both gave iced up drops (I
only tried quick and dirty tests by dipping a cover glass in liquid
nitrogen).
Thank you.
-Chris
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