I always try sugars for everything. There is a cryocrystallography webinar
at rigaku.com with embedded videos on how to do this. 50% to 100% saturated
sugar (sucrose, glucose, trehalose, sorbitol, et al.) in reservoir buffer is
usually what I try. :)
-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Chris
Ulens
Sent: Thursday, May 26, 2011 5:27 AM
To: [log in to unmask]
Subject: Re: [ccp4bb] recommendation for ammonium dihydrogen phosphate cryo
Hi,
I would like to get recommendations for a proper cryo solution for a
crystallization hit from the Hampton crystal screen Ammonium di- hydrogen
phosphate 0.4M. I tried increasing glycerol up to 30% with the same ammonium
phosphate concentration or increasing glycerol up to 30% in the presence of
1.3M ammonium phosphate. Both gave iced up drops (I only tried quick and
dirty tests by dipping a cover glass in liquid nitrogen).
Thank you.
-Chris
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