Hi Joane,
since you're using NCS I'd like to add that this is indeed a good
strategy, but you should make sure that you should probably not restrain
the B-factors of the well-defined domain and the not-so-well-defined
domains to be equal - they do differ and the refinement should probably
not change that!
So I'd say: adjust the weights such that the coordinates are
NCS-restrained, but not the B-factors (or only a little). Use R-free to
find the right values for the restraints.
HTH,
Kay
--
Kay Diederichs http://strucbio.biologie.uni-konstanz.de
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Fachbereich Biologie, Universität Konstanz, Box M647, D-78457 Konstanz
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