[switch not too serious mode on]:
well, it is lysozyme, which, according to diffraction properties, should perhaps be classified as a salt (LyCl7), not a protein... :-)
Mark J van Raaij
Laboratorio M-4
Dpto de Estructura de Macromoleculas
Centro Nacional de Biotecnologia - CSIC
c/Darwin 3, Campus Cantoblanco
E-28049 Madrid, Spain
tel. (+34) 91 585 4616
http://www.cnb.csic.es/content/research/macromolecular/mvraaij
On 4 May 2011, at 17:53, Dhanasekaran Varudharasu wrote:
> Dear all,
>
> We have solved the sturcture of hen egg white lysozyme with a barium ion at 2.7 fold data redundancy. Data collected at in-house copper K-alpha source to 2.22 A resolution with 1 degree oscillation step per frame. The substructure was correctly identified with just 8 frames ( up to 7 frams the SHELXC did not produced the hkl files) when using SHELXD and phasing was successful when using SHELXE-Beta trail version with 35 frames. We can not belive it because the data used for substructure solution has only 37% completeness. The crystallographers frequently says that in SAD phasing to find the correct anomalous substructe the data reduendancy should be high enough. But in our case the SHELXD was identified the heavy atom site correctly at this very low completeness.
>
> I welcome your suggestions and comments.
>
> Thanks
> with regards
> Dhana
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