Hi,
Yes indeed, as Matt was kind enough to point out, we do have the HC1 installed at station I911-3 of the MAX-II ring and you are welcome to make a rapid access application. One advantage of MAX-lab might be that our wiggler beam is more merciful to the crystals at room temperature than the undulator beams at the ESRF! There are a few free days left in our spring schedule:
http://cassiopeia.maxlab.lu.se/index/organiser-app
Good luck
Derek
___________________________________________________________________
Derek Logan tel: +46 46 222 1443
Associate Professor fax: +46 46 222 4692
Dept. of Biochemistry and Structural Biology mob: +46 76 8585 707
Centre for Molecular Protein Science www.cmps.lu.se
Lund University, Box 124, 221 00 Lund, Sweden www.saromics.com
On May 2, 2011, at 8:11, Matthew BOWLER wrote:
> Dear Israel,
> as Martin pointed out we have a device here at the ESRF/EMBL, the
> HC1b, that produces a stream of air with a precisely controlled RH at
> the sample position that we have used with some success to monitor the
> effects dehydration has on diffraction quality. The same device is also
> available at Diamond, Max-Lab and, I believe, BESSY. The example you
> describe is a classic example of the sort of system that will usually
> benefit from controlled dehydration. Depending on the size and
> concentration of the LMW PEG you are using you have probably reduced the
> "RH" surrounding your crystal by ~10%. The best thing to do now is
> repeat these experiments using the HC1b to really define the changes in
> the lattice of your crystals and find the optimum dehydration conditions
> for your crystals. At the ESRF the device can be requested for any
> experimental session (just click the check box on the A form) and I
> presume that this will be similar at the other synchrotrons.
>
> As well as the reference describing the device we have recently
> published a further description of typical experimental conditions and
> some successful applications:
>
> http://dx.doi.org/10.1016/j.jsb.2011.03.002
>
> And the ESRF webpage is here:
>
> http://www.esrf.fr/UsersAndScience/Experiments/MX/About_our_beamlines/ID14-2/HC1b
>
> Good luck! Matt
>
>
> On 01/05/2011 19:32, Israel Sanchez wrote:
>> Hi folks,
>>
>>
>> I am currently impressed by the efficiency of dehydration treatments
>> over the diffraction capacity of our crystals in one particular
>> condition. Without any treatment the crystals seldom diffract to
>> 20-30A but in our last synchrotron trip the very same crystals, after
>> been incubated with increasing concentration of low molecular weight
>> PEGs diffracted to 6A.
>>
>> I was wondering if anyone has studied these effects in a systematic
>> way. Does anyone on the ccp4bb knows references or has any
>> experience/pseudo-religious believes that do not care to share with
>> the community about this particular topic?
>>
>>
>> Thank you very much in advance!!!!
>>
>>
>> --
>> Israel Sanchez Fernandez PhD
>> Ramakrishnan-lab
>> MRC Laboratory of Molecular Biology,
>> Hills Road, Cambridge, CB2 0QH, UK
>>
>>
>
> --
> Matthew Bowler
> Structural Biology Group
> European Synchrotron Radiation Facility
> B.P. 220, 6 rue Jules Horowitz
> F-38043 GRENOBLE CEDEX
> FRANCE
> ===================================================
> Tel: +33 (0) 4.76.88.29.28
> Fax: +33 (0) 4.76.88.29.04
>
> http://go.esrf.eu/MX
> http://go.esrf.eu/Bowler
> ===================================================
|