Hi Pengfei,
Yes, that's one thing scaling takes care of.
Cheers, Tim
On Tue, May 31, 2011 at 02:59:00AM +0800, Pengfei Fang wrote:
> Hi Tim,
>
> I am worry about if there is any problem just scale the two sets together in HKL2000, since they had different exposure time.
> Will the program match them?
>
> thanks,
> Pengfei
>
>
>
>
> At 2011-05-31 02:50:41,"Tim Gruene" <[log in to unmask]> wrote:
>
> >Dear pengfei,
> >
> >you do not need to use mosflm if you want to scale the datasets with scala. You
> >can use scalepack2mtz (available from ccp4i -> Program List) to convert the two
> >sca-files to mtz-format and then use scala for scaling.
> >Maybe HKL2000/scalepack can scale the files for you if you integrate them both
> >in the same run (load both data sets, and HKL2000 will integrate both data
> >sets).
> >
> >Tim
> >
> >On Tue, May 31, 2011 at 02:40:49AM +0800, Pengfei Fang wrote:
> >> Hi David,
> >>
> >> Thanks for your help. I just scaled the two data sets independently using HKL2000.
> >> And, I don't know how to set reference in HKL2000 or CCP4.
> >> If using CCP4, should I also use imosflm to do integrate?
> >>
> >> thanks,
> >> pengfei
> >>
> >>
> >> At 2011-05-31 02:32:03,"David Veesler" <[log in to unmask]> wrote:
> >> Hi Pengfei,
> >> you can combine your files using pointless but it is unclear to me based on your message if you have scaled independently the two data sets or not.
> >> You should scale these two data sets together using one as reference (doable using XSCALE or SCALA...).
> >> Cheers
> >> David
> >>
> >>
> >> Le 30 mai 2011 à 11:13, Pengfei Fang a écrit :
> >>
> >> Dear All,
> >>
> >> I have two data sets from one crystal.
> >> One had short exposures to keep the low resolution reflections from saturating. The other had long exposure to get high resolution data.
> >> I am wondering how two merge these two data sets into one file that I can use for refinement.
> >>
> >> The two sets already scaled by HKL2000. Now I have two .sca files.
> >>
> >> Thanks a lot!
> >> Pengfei Fang
> >>
> >>
> >>
> >> --
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> >--
> >--
> >Tim Gruene
> >Institut fuer anorganische Chemie
> >Tammannstr. 4
> >D-37077 Goettingen
> >
> >phone: +49 (0)551 39 22149
> >
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--
--
Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
phone: +49 (0)551 39 22149
GPG Key ID = A46BEE1A
|