Hello,
Thanks, that is indeed correct, Tim spotted this last night but I forgot
to forward to the list. This is something that Rasmus will be best placed
to fix and he's not in today (and I can't remember if he is back this week
or not, but I thought he would be back today so that was already wrong).
Wayne
On Tue, 26 Apr 2011, Fowler, Andrew wrote:
> Sorry to reply to my own message from yesterday, but after a bit of poking
> around I think I've found the problem with making distance restraints from my
> double filtered NOESY.
>
> Before changing the experiment to the "wrong" type and restricting the residue
> range as a workaround, I decided to take a look at the experiment prototypes to
> see if there was something closer and found the problem, which can be seen in
> the attached image.
>
> Briefly, the experiment I need is defined correctly as prototype 75. A double
> filtered TOCSY experiment is later defined as prototype 225, but the synonym is
> incorrectly set as a NOESY – when picking this synonym in the Experiment window,
> it only allows one to select the highest number/most recent full type. If I
> create a copy of the correct definition (since the stock prototypes can't be
> edited), then when I select the correct synonym in the Experiment window it sets
> the correct full type automatically and the experiment is available in Make
> Distance Restraints.
>
> It looks to me like a permanent fix involves simply fixing the synonym for
> prototype 225. Wayne – could you please make this change?
>
> Cheers,
> Andrew
>
> --
>
> From: Andrew Fowler <[log in to unmask]>
> Reply-To: "CcpNmr software mailing list <[log in to unmask]>"
> <[log in to unmask]>
> Date: Mon, 25 Apr 2011 20:09:15 +0000
> To: "CcpNmr software mailing list <[log in to unmask]>"
> <[log in to unmask]>
> Subject: Another Make Distance Restraints Issue
>
> I've encountered another problem as I make my way through making my
> distance restraints.
>
> I'm trying to make intramolecular NOESY restraints for the unlabeled
> peptide in my complex. Unfortunately, peaklists for my doubly filtered
> NOESY experiments don't show up under M:Structure:Make Distance
> Restraints.
>
> I'm not sure if this is the problem, but wondering if I had defined the
> experiment type correctly for this experiment, I took a look at the
> Experiment Types panel - see attached image. While the type synonym is
> correct (12CH/14NH -> 12CH/14NH NOESY), the full type shows up as a TOCSY,
> not a NOESY, and is the only available option.
>
> If I change the experiment type to 12CH/14NH -> H NOESY, the full type
> changes to a NOESY and the experiment shows up in Make Distance
> Restraints. However, the second dimension in this experiment is then not
> filtered (although I suppose I can restrict the residue range to chain B
> to keep working for now).
>
> This is Analysis 2.1.5 updated sometime last week if it matters.
>
> Andrew
>
>
>
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