We had a case with a BME adduct clearly seen in the structure.
PDB ID 1CY5.
Leemor
On 4/15/11 4:09 PM, "Michael Thompson" <[log in to unmask]> wrote:
> Hi All,
>
> I was wondering if anyone knew whether or not it is possible for reducing
> agents with thiol groups, such as DTT or beta-mercaptoethanol (BME), to form
> covalent S-S bonds with Cys residues, particularly solvent-exposed Cys? I have
> some puzzling biochemical results, and in the absence of a structure (thus
> far), I was wondering if this might be something to try to control for. I have
> never heard of this happening (or seen a structure where there was density for
> this type of adduct), but I can't really think of a good reason for why this
> wouldn't happen. Especially for something like BME, where the molecule is very
> much like the Cys sidechain and seems to me like it should have similar
> reactivity. The only thing I can think of is if there is a kinetic effect
> taking place. Perhaps the rate of diffusion of these small molecules is much
> faster that the formation of the S-S bond?
>
> Does anyone know whether or not this is possible, and why it does or does not
> happen?
>
> Thanks,
>
> Mike
>
>
>
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