PS, it might be a good time to start an additive screen.
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From: "Jun Yong Ha" <[log in to unmask]>
Sent: Tuesday, April 12, 2011 7:56 AM
To: <[log in to unmask]>
Subject: [ccp4bb] Reproducing crystals.
> Hi all,
>
> Recently, I produced crystals with MBClass1-64 which contains PEG4000,
> HEPES-Na and NaCl. But, I struggled to reproduce crystals. I tried to set
> up tray with different batch of solution. I got the crystals only from
> 2008 solution, but not from fresh ones. I asked technical service of
> Qiagen, but they did not have any stock.
>
> pH between fresh and old solution is the same. I could reproduce crystals
> with this old solution 100% when setting up.
>
> Do you have any experience like this? Is PEG4000 degraded or oxidized?
>
> Please help me.
>
> Thanks in advance.
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