Related fact I learned recently, perhaps of interest for fellow record-keepers:
heparin sulfate has the highest negative charge density of any known
biological molecule.
JPK
On Sat, Apr 9, 2011 at 9:38 PM, Raji Edayathumangalam <[log in to unmask]> wrote:
> Hi Alex,
>
> Most DNA-binding proteins has decent affinity to heparin columns. Have you
> tried purifying your protein over heparin columns?
>
> Cheers,
> Raji
>
>
>
> On Sat, Apr 9, 2011 at 8:44 PM, Alexandra Deaconescu <[log in to unmask]>
> wrote:
>>
>> Hello ccp4 enthusiasts:
>>
>> I am afraid this is a non-ccp4 related question. Can anyone recommend an
>> immobilized dsDNA chromatographic resin for purification of DNA-binding
>> proteins? GE seems to have something - I was wondering if people have other
>> recommendations? In the age of GST and His tags etc., these are not very
>> much used, but I do not have a tag in this case...
>>
>> Thanks a lot,
>> Alex
>
>
>
> --
>
> -----------
> Raji Edayathumangalam
> Research Fellow in Neurology, Harvard Medical School
> Postdoctoral Fellow, Center for Neurologic Diseases, Brigham and Women's
> Hospital
> Visiting Research Scholar, Brandeis University
>
>
--
*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
cel: 773.608.9185
email: [log in to unmask]
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