I should say clearer that I did a comparison between GE (Superdex 200
10/30) and the Wyatt column of the same separation range on AKTA
connected with MALS. The Wyatt column gave better resolution for size
exclusion chromatography as well as signal-noise ratio for light
scattering. So I think it is worth for you to try it. They offer
money-back guarantee.
Best wishes,
Sally.
On Tue, Mar 8, 2011 at 3:36 PM, Sally Pham Thanh Van
<[log in to unmask]> wrote:
> Dear Sebastiano,
>
> From my experiences, Wyatt columns are the best.
>
> Best wishes,
> Sally.
>
> On Tue, Mar 8, 2011 at 2:03 PM, Sebastiano Pasqualato
> <[log in to unmask]> wrote:
>> Dear all,
>> I was wondering if somebody could help me out by suggesting the "best" column to be used in a Static Light Scattering (I guess it would be the same for a Multi Angle Light Scattering) instrument.
>> We were suggested using a silica-based column, with very high separation properties, but it seems that these columns are highly sensitive to (even slightly) basic pH's. Even running the column in PBS, it looks like injecting samples at pH 8.0 ruins the column resin, making it unusable.
>> On the other hand, GE Healthcare columns would require a huge amount of material to be loaded.
>> What are you guys using in your instruments?
>> Thanks a lot in advance for the feedback,
>> best,
>> ciao
>> Sebastiano
>>
>> --
>> Sebastiano Pasqualato, PhD
>> Crystallography Unit
>> IFOM-IEO Campus
>> Cogentech - Consortium for Genomic Technologies
>> via Adamello, 16
>> 20139 - Milano
>> Italy
>>
>> tel +39 02 9437 5172
>> fax +39 02 9437 5990
>>
>
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