afaik Mosflm will index on whatever spots you have told it to find and not discard, be they from 1 image, 2, or 10 or more images at any relative angle or oscillation range. Or it won't successfully index, depending on the difficulty of the case (several lattices, spot overlap etc...).
Indexing from two images at 90 degrees from each other is simply an easy "good practice" rule that often, but not absolutely always, works.
Many times a single image is more than enough, sometimes many images and a lot of trying is necessary to get the right indexing matrix.
Mark J van Raaij
Laboratorio M-4
Dpto de Estructura de Macromoleculas
Centro Nacional de Biotecnologia - CSIC
c/Darwin 3, Campus Cantoblanco
E-28049 Madrid, Spain
tel. (+34) 91 585 4616
http://www.cnb.csic.es/content/research/macromolecular/mvraaij/index.php?l=1
On 17 Mar 2011, at 19:44, Bryan Lepore wrote:
> I seem to have noticed that (i)mosflm can index on one image, or two
> images that are not related by 90 degrees. also, cell refinement
> sometimes splits up a set of frames into maybe 3 or four segments of
> e.g. a few degrees each. and of course, I can set it based on frames
> 90 degrees apart.
>
> reason I ask is that i thought mosflm's modus operandi was based on
> frames in general being related by 90 degrees - is mosflm simply
> written differently or is there some criteria it uses to make these
> settings, or something else...
>
> -Bryan
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