On Wed, 2011-03-09 at 10:52 +0000, Art wrote:
> No protein crystal was found after some typical conditions (kit I, kit
> II, and Index) were repeatly screened for more than three times.
See Einstein's definition of insanity :)
> Protein concentration gradient was considered, but it can not work.
Not sure what you mean by this, but you can also try diluting reservoir
solutions. Also, given your protein buffer, you may be more or less
locking yourself into a narrow pH range - try 10mM MES instead.
--
"I'd jump in myself, if I weren't so good at whistling."
Julian, King of Lemurs
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