Dear Sebastiano,
From my experiences, Wyatt columns are the best.
Best wishes,
Sally.
On Tue, Mar 8, 2011 at 2:03 PM, Sebastiano Pasqualato
<[log in to unmask]> wrote:
> Dear all,
> I was wondering if somebody could help me out by suggesting the "best" column to be used in a Static Light Scattering (I guess it would be the same for a Multi Angle Light Scattering) instrument.
> We were suggested using a silica-based column, with very high separation properties, but it seems that these columns are highly sensitive to (even slightly) basic pH's. Even running the column in PBS, it looks like injecting samples at pH 8.0 ruins the column resin, making it unusable.
> On the other hand, GE Healthcare columns would require a huge amount of material to be loaded.
> What are you guys using in your instruments?
> Thanks a lot in advance for the feedback,
> best,
> ciao
> Sebastiano
>
> --
> Sebastiano Pasqualato, PhD
> Crystallography Unit
> IFOM-IEO Campus
> Cogentech - Consortium for Genomic Technologies
> via Adamello, 16
> 20139 - Milano
> Italy
>
> tel +39 02 9437 5172
> fax +39 02 9437 5990
>
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