No - and I dont think it is accepted practice now either..
I often use I/SigI > 1.5 for refinement..
Look at your Rfactor plots from REFMAC - if they look reasonable at
higher resolution use the data
Eleanor
On 03/03/2011 11:29 AM, Roberto Battistutta wrote:
> Dear all,
> I got a reviewer comment that indicate the "need to refine the structures at an appropriate resolution (I/sigmaI of>3.0), and re-submit the revised coordinate files to the PDB for validation.". In the manuscript I present some crystal structures determined by molecular replacement using the same protein in a different space group as search model. Does anyone know the origin or the theoretical basis of this "I/sigmaI>3.0" rule for an appropriate resolution?
> Thanks,
> Bye,
> Roberto.
>
>
> Roberto Battistutta
> Associate Professor
> Department of Chemistry
> University of Padua
> via Marzolo 1, 35131 Padova - ITALY
> tel. +39.049.8275265/67
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> www.chimica.unipd.it/roberto.battistutta/
> VIMM (Venetian Institute of Molecular Medicine)
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