Pius,
Are you sure that you determined the correct cell. Which program did you
use? Usually there are much less spots on an image when a crystal has so
small unit size dimensions. To me the first crystal looks like protein.
Send it to a synchrotron and process the data in XDS. They can process
for you.
Maia
On 21/03/2011 4:33 PM, Maia Cherney wrote:
> Hi PS
>
> What is the unit cell dimensions in the first crystal? It looks like
> protein to me.
>
> Maia
>
>
>
> On 21/03/2011 2:03 PM, Pius Padayatti wrote:
>> Hi all,
>> We recently observed some diffraction from membrane protein
>> crystallization
>> drops diffraction that look like non-proteinaceous (please see
>> attached files,
>> from 4 different crystals grown in different conditions).
>> Rains' question about about lipid and detergent diffraction is so
>> relevant.
>>
>> This is most likely what lipids and detergent diffraction looks like?
>> People with similar experience and know what could be these patterns
>> might be from may have better suggestions and
>> would like to hear all comments.
>>
>> first four images are from drops where detergent is DDM and and vapor
>> diffusion
>> while last image is from a crystal grown in mesophase (with monoolein).
>>
>> Padayatti PS
>>
>>
>> On Sat, Mar 19, 2011 at 7:19 PM,<Rain Field> <[log in to unmask]>
>> wrote:
>>> Hi All,
>>> I am wondering if the detergent or lipid crystal can have
>>> diffraction at low resolution.
>>> If they can, what does the diffraction pattern looks like? Are there
>>> any literatures describing these?
>>> Many thanks!
>>>
>
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