Well BR, do not underestimate complexity of running a gel! There are even more harsh referees comments on gel appearance and quality
than comments on cutting data based on R,RF and sigmaI :-)
Especially when one is trying to penetrate into prestigious journals...
Dr Felix Frolow
Professor of Structural Biology and Biotechnology
Department of Molecular Microbiology
and Biotechnology
Tel Aviv University 69978, Israel
Acta Crystallographica F, co-editor
e-mail: [log in to unmask]
Tel: ++972-3640-8723
Fax: ++972-3640-9407
Cellular: 0547 459 608
On Mar 3, 2011, at 18:38 , Bernhard Rupp (Hofkristallrat a.D.) wrote:
>> related to what I feel is recent revival of the significance of the R-values
>
> ....because it's so handy to have one single number to judge a highly complex nonlinear multivariate barely determined regularized problem! Just as easy as running a gel!
>
> Best BR
>
> -----Original Message-----
> From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Ed Pozharski
> Sent: Thursday, March 03, 2011 8:19 AM
> To: [log in to unmask]
> Subject: Re: [ccp4bb] I/sigmaI of >3.0 rule
>
> On Thu, 2011-03-03 at 08:08 -0700, Bart Hazes wrote:
>> I don't know what has caused this wave of high I/Sigma threshold use
>> but here are some ideas
>>
>
> It may also be related to what I feel is recent revival of the significance of the R-values in general. Lower resolution cutoffs in this context improve the R-values, which is (incorrectly) perceived as model improvement.
>
> --
> "I'd jump in myself, if I weren't so good at whistling."
> Julian, King of Lemurs
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