Working with the H+-ATPase AHA2 (C12E8/DDM/Cymal-5 detergent mixed
micelle) we always saw a ring at ~40Å which we attributed to the
micelle, but we have no experimental evidence that it was indeed caused
by detergents.
Heres a animated gif of this phenomenon:
http://www.bioxray.au.dk/~bjopp/pictures/zoom.gif
Also recently I tested some very nice looking and surprisingly hexagonal
xtal-like structures in a drop full of DDM crystals. I got this '6-fold'
pattern:
http://www.bioxray.au.dk/~bjopp/pictures/ddm_diff.png
Again I'm guessing detergent, but I would like to hear any alternative
explanations. :)
hth
-Bjørn
--
Bjørn Panyella Pedersen, PhD
Postdoc
Macromolecular Structure Group
Dept. of Biochemistry and Biophysics
University of California, San Francisco
MC2240, 600 - 16th Street S414
San Francisco, CA 94158-2517
Phone: +1 415-476-3937
E-mail: [log in to unmask]
http://www.msg.ucsf.edu
On 2011-03-19 16:19, <Rain Field> wrote:
> Hi All,
> I am wondering if the detergent or lipid crystal can have diffraction at low resolution.
> If they can, what does the diffraction pattern looks like? Are there any literatures describing these?
> Many thanks!
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