On behalf of the P-cube management team:
-Peer
Dear All,
Don't miss out on the P-CUBE workshop in Oxford in April 2011! Register
today under www.p-cube.eu and learn everything about mammalian
expression technologies.
The registration deadline is March 19, 2011.
See you in Oxford!
P-CUBE Management Team
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P-CUBE Workshop on Mammalian Expression Technologies
Oxford, 3-8 April 2011
The course is designed primarily for structural biologists who have
prokaryotic expression experience and would like to use the mammalian
expression system for more challenging targets. The goals of the course
are to allow participants to have hands-on experience of mammalian cell
culture, small and large scale transient gene expression including the
use of automated systems, protein purification and crystallization. The
course will be structured so as to interleave lectures and practical
sessions. The participants will have the opportunity to closely interact
with the tutors and to directly discuss their research projects with the
experts in small workgroups.
PARTICIPANTS
Participants will be asked to submit their CV and a letter of
motivation. The course will be limited to 12 participants from European
structural biology laboratories.
• There is no registration fee for the workshop.
• Accommodation (5 nights max.) during the workshop will be covered.
• Participants are responsible for their own travel costs.
TOPICS:
1. Mammalian expression platform overview
2. Mammalian cell lines for protein expression and glycosylation control
3. Mammalian expression constructs design
4. Small scale expression screen with Western Blot
5. Large scale expression and protein purification
6. Protein crystallization
7. Protein structure case studies
SPEAKERS:
Radu Aricescu (Oxford University, UK), Yuguang Zhao (Oxford University,
UK), Joop van den Heuvel (Helmholtz-Zentrum für Infektionsforschung,
DE), Veronica Chang (Oxford University, UK), Ben Bishop (Oxford
University, UK), Karl Harlos (Oxford University, UK), Tom Walter (Oxford
University, UK), Christian Siebold (Oxford University, UK), Joerg
Standfuss (Paul Scherrer Institut, CH)
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