The Catalase tetramer is around there (232 kD according to the Table 1 in Mylonas and Svergun, J. Applied Cryst. 2007). The shape of the tetramer in the PDB (4BLC) seems reasonably globular.
Cheers,
Kushol
Kushol Gupta, Ph.D.
Research Associate
Van Duyne Laboratory - HHMI/Univ. of Pennsylvania School of Medicine
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215-573-7260 / 267-259-0082
-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Alexandra Deaconescu
Sent: Saturday, February 19, 2011 7:47 PM
To: [log in to unmask]
Subject: [ccp4bb] 250 kDa standard
Dear ccp4bb enthusiasts:
A question unrelated to ccp4: can anyone recommend a good 250 kDa
standard for gel filtration that is commercially available? It could be
a single polypeptide or an oligomer too...
Thanks a lot!
Bests,
Alex
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